http://bolin.su.se/data/oden-ao-2018-mart-dna-1 Jessica A. Mirrielees, Rachel M. Kirpes, Patricia A. Matrai, Kerri A. Pratt Bolin Centre Database 2024 Datafile Atmosphere Ecosystems Ocean biology and chemistry Marine Marine aerosol reference tank Marine microbial community 16S DNA 18S DNA AO2018 AO18 MOCCHA Icebreaker Oden Earth science > Biosphere > Aquatic ecosystems > Plankton Jessica Mirrielees 2024-06-05T05:22:31+00:00 English 1 The data are provided in one comma-separated values (csv) file (~2 kB). File structure (columns) and contents: 1. name of experiment (description of water sampling location) 2. date (YYYY-MM-DD) 3. time (when surface water was sampled) in UTC (HH:MM) 4. latitude (water sampling location) 5. longitude (water sampling location) 6. relative sequence abundance of chlorophyta (18S) 7. relative sequence abundance of haptophyta (18S) 8. relative sequence abundance of bacillariophyta (18S) 9. relative sequence abundance of dinoflagellata (18S) 10. relative sequence abundance of holozoa (18S) 11. relative sequence abundance of ciliophora (18S) 12. relative sequence abundance of cercozoa (18S) 13. sum of relative sequence abundance of cryptomonads, fungi, and minor/miscellaneous taxonomic groups (18S) 14. relative sequence abundance of Bacteroidia (16S) 15. relative sequence abundance of Alphaproteobacteria (16S) 16. relative sequence abundance of Gammaproteobacteria (16S) 17. sum of relative sequence abundance of minor/miscellaneous taxonomic groups (16S) Samples were collected for 16S and 18S DNA analysis on 0.2 μm Nucleopore membrane filters. Following collection, these samples were immediately frozen in liquid nitrogen and stored at −80 °C until extraction. Samples were extracted and purified with DNeasy® PowerWater® (Qiagen), and sent to Integrated Microbiome Resource (IMR) at Dalhousie University (Halifax, Canada) for gene amplification, sequencing, and community composition assignments. Taxonomic classification was assigned using the [Microbiome Helper pipeline](https://github.com/LangilleLab/microbiome_helper/wiki), based on QIIME2, that uses Amplicon Sequence Variants (ASVs) created with Deblur. Operational taxonomic units were then grouped mostly at the phylum level, following the [National Center for Biotechnology Information (NCBI) Taxonomy Browser](https://www.ncbi.nlm.nih.gov/). The marine aerosol reference tank experiments were carried out during the Arctic Ocean 2018 expedition on board the Swedish icebreaker Oden, which was made in collaboration between Sweden and the US National Science Foundation (NSF) and organized by the [Swedish Polar Research Secretariat](https://polarforskningsportalen.se/en/arctic/expeditions/arctic-ocean-2018). The ship track with latitude and longitude information can be found in the [Navigation, meteorological and surface seawater data from the Arctic Ocean 2018 expedition](https://bolin.su.se/data/oden-ao-2018-navigation-1) data set.