[{"name":"oden-ao-2018-mart-dna-1","title":"18S and 16S DNA analysis of surface water used in the Arctic Ocean 2018 marine aerosol reference tank experiments","summary":"This dataset includes the relative sequence abundance of several taxonomic groups in seawater collected during the Arctic Ocean 2018 expedition to the central Arctic Ocean in August and September 2018. Taxonomic groups were assigned using 16S (prokaryotes) and 18S DNA (eukaryotes) analysis.\r\n\r\nA series of 10 aerosol generation experiments were carried out using locally collected surface water from various locations in the Arctic, including the marginal ice zone, the North Pole, several open lead sites, and a melt pond on an ice floe. The MART employed a plunging jet mechanism to generate sea spray aerosol particles.\r\n\r\nDuring the first nine experiments, prior to aerosol generation, a subsample of the surface water was filtered (to collect cells greater than 0.2 \u03bcm in diameter), then frozen and stored. The 16S and 18S DNA analysis was subsequently carried out on subsample from each experiment in order to explore the relationship between marine biology and aerosol particle size\/composition. 16S and 18S DNA analysis was not carried out for the filtered seawater experiment.","citations":"","comments":"Samples were collected for 16S and 18S DNA analysis on 0.2 \u03bcm Nucleopore membrane filters. Following collection, these samples were immediately frozen in liquid nitrogen and stored at \u221280 \u00b0C until extraction.\r\n\r\nSamples were extracted and purified with DNeasy\u00ae PowerWater\u00ae (Qiagen), and sent to Integrated Microbiome Resource (IMR) at Dalhousie University (Halifax, Canada) for gene amplification, sequencing, and community composition assignments. Taxonomic classification was assigned using the [Microbiome Helper pipeline](https:\/\/github.com\/LangilleLab\/microbiome_helper\/wiki), based on QIIME2, that uses Amplicon Sequence Variants (ASVs) created with Deblur.\r\n\r\nOperational taxonomic units were then grouped mostly at the phylum level, following the [National Center for Biotechnology Information (NCBI) Taxonomy Browser](https:\/\/www.ncbi.nlm.nih.gov\/).\r\n\r\nThe marine aerosol reference tank experiments were carried out during the Arctic Ocean 2018 expedition on board the Swedish icebreaker Oden, which was made in collaboration between Sweden and the US National Science Foundation (NSF) and organized by the [Swedish Polar Research Secretariat](https:\/\/polarforskningsportalen.se\/en\/arctic\/expeditions\/arctic-ocean-2018).\r\n\r\nThe ship track with latitude and longitude information can be found in the [Navigation, meteorological and surface seawater data from the Arctic Ocean 2018 expedition](https:\/\/bolin.su.se\/data\/oden-ao-2018-navigation-1) data set.","category":"Atmosphere","subcategory":"Ecosystems","keywords":"Ocean biology and chemistry; Marine; Marine aerosol reference tank; Marine microbial community; 16S DNA; 18S DNA; AO2018; AO18; MOCCHA; Icebreaker Oden","scientist":"Jessica A. Mirrielees, Rachel M. Kirpes, Patricia A. Matrai, Kerri A. Pratt","firstname":"Jessica","lastname":"Mirrielees","address":"Department of Chemistry; University of Michigan","postalcode":"MI 48109-1055","city":"Ann Arbor","province":"Michigan","country":"USA ","parameters":"Earth science > Biosphere > Aquatic ecosystems > Plankton","location":"Ocean > Arctic Ocean","progress":"","language":"English","project":"Arctic Ocean 2018. The 'Aerosol-cloud interactions in the High Arctic' project during the Arctic Ocean 2018 expedition was supported by the Knut-and-Alice-Wallenberg Foundation within the ACAS project (Arctic Climate Across Scales, project no. 2016.0024), the Bolin Centre for Climate Research (RA2), and the Swedish Research Council (project no. 2018-05045 and project no. 2016-05100).","publisher":"Bolin Centre Database","version":"1","constrains":"","access":""}]