18S and 16S DNA analysis of surface water used in the Arctic Ocean 2018 marine aerosol reference tank experiments

Jessica A. Mirrielees, Rachel M. Kirpes, Patricia A. Matrai, Kerri A. Pratt

This dataset includes the relative sequence abundance of several taxonomic groups in seawater collected during the Arctic Ocean 2018 expedition to the central Arctic Ocean in August and September 2018. Taxonomic groups were assigned using 16S (prokaryotes) and 18S DNA (eukaryotes) analysis.

A series of 10 aerosol generation experiments were carried out using locally collected surface water from various locations in the Arctic, including the marginal ice zone, the North Pole, several open lead sites, and a melt pond on an ice floe. The MART employed a plunging jet mechanism to generate sea spray aerosol particles.

During the first nine experiments, prior to aerosol generation, a subsample of the surface water was filtered (to collect cells greater than 0.2 μm in diameter), then frozen and stored. The 16S and 18S DNA analysis was subsequently carried out on subsample from each experiment in order to explore the relationship between marine biology and aerosol particle size/composition. 16S and 18S DNA analysis was not carried out for the filtered seawater experiment.

Citation

Jessica A. Mirrielees, Rachel M. Kirpes, Patricia A. Matrai, Kerri A. Pratt (2024) 18S and 16S DNA analysis of surface water used in the Arctic Ocean 2018 marine aerosol reference tank experiments. Dataset version 1. Bolin Centre Database. https://doi.org/10.17043/oden-ao-2018-mart-dna-1

Data description

The data are provided in one comma-separated values (csv) file (~2 kB).

File structure (columns) and contents:

1. name of experiment (description of water sampling location)
2. date (YYYY-MM-DD)
3. time (when surface water was sampled) in UTC (HH:MM)
4. latitude (water sampling location)
5. longitude (water sampling location)
6. relative sequence abundance of chlorophyta (18S)
7. relative sequence abundance of haptophyta (18S)
8. relative sequence abundance of bacillariophyta (18S)
9. relative sequence abundance of dinoflagellata (18S)
10. relative sequence abundance of holozoa (18S)
11. relative sequence abundance of ciliophora (18S)
12. relative sequence abundance of cercozoa (18S)
13. sum of relative sequence abundance of cryptomonads, fungi, and minor/miscellaneous taxonomic groups (18S)
14. relative sequence abundance of Bacteroidia (16S)
15. relative sequence abundance of Alphaproteobacteria (16S)
16. relative sequence abundance of Gammaproteobacteria (16S)
17. sum of relative sequence abundance of minor/miscellaneous taxonomic groups (16S)

Comments

Samples were collected for 16S and 18S DNA analysis on 0.2 μm Nucleopore membrane filters. Following collection, these samples were immediately frozen in liquid nitrogen and stored at −80 °C until extraction.

Samples were extracted and purified with DNeasy® PowerWater® (Qiagen), and sent to Integrated Microbiome Resource (IMR) at Dalhousie University (Halifax, Canada) for gene amplification, sequencing, and community composition assignments. Taxonomic classification was assigned using the Microbiome Helper pipeline, based on QIIME2, that uses Amplicon Sequence Variants (ASVs) created with Deblur.

Operational taxonomic units were then grouped mostly at the phylum level, following the National Center for Biotechnology Information (NCBI) Taxonomy Browser.

The marine aerosol reference tank experiments were carried out during the Arctic Ocean 2018 expedition on board the Swedish icebreaker Oden, which was made in collaboration between Sweden and the US National Science Foundation (NSF) and organized by the Swedish Polar Research Secretariat.

The ship track with latitude and longitude information can be found in the Navigation, meteorological and surface seawater data from the Arctic Ocean 2018 expedition data set.